Technical Research Assistant I BWH Neurology
Brigham & Women's Hospital(BWH)

Job Info

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GENERAL SUMMARY/OVERVIEW STATEMENT:

Works independently, under minimal supervision. Executes protocols involving a variety of routine and non-routine experiments at a professional level. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to research design. Calculates, transcribes and graphs data. Trains new staff and may serve in a limited supervisory role.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

• Under general supervision breeds and maintains common use mouse strains and use molecular techniques (PCR, quantitative PCR) and cellular techniques (flow cytometry) to genotype transgenic and knockout mice.
• Maintains basic records of mouse breeding, methodologies and test results in a log of work performed.
• Performs basic cell culture, primary neuron culture and in vitro (e.g. LDH, enzymatic) assays and examination of protein expression level by ELISA, immunoblots, intracytoplasmic staining, and cytometric bead array.
• Performs mouse brain dissection, RNA or protein quantification, tissue RNA or protein isolation and RNA or protein quality analysis by commercially available kits and protocols.
• Performs in vivo experiments involving hands-on behavioral studies such as pole testing, open field testing, water maze testing and clasping scores.
• Assists in the orientation and training of new staff in established laboratory techniques.
• Coordinates daily schedule of experiments.
• Responsible for data management: collects, records, tabulates and presents data. Creates graphs of experimental, performs statistical tests, and discusses progress of work regularly with supervisor.
• All other duties as assigned.
  

Qualifications
QUALIFICATIONS:

• Must have a Bachelor's degree and at least one-year experience in a directly related research setting.
• Must have experience in wet labs and with mouse work (preferred in sections mentioned in "principal duties and responsibilities")

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

• High degree of computer literacy
• Sound analytical and organizational skills.
• Good oral and written communication skills.
• Structures logically and effectively tasks and set priorities
• Is able to identify potential problems and trouble-shoot solutions.
• Demonstrates ability to analyze data statistically and to logically present it.
• Shows strong interpersonal communication skills.

WORKING CONDITIONS:

• Normal laboratory environment.
• Hazardous materials are used in some of the experiments conducted.
• Animal tissue will be used.

SUPERVISORY RESPONSIBILITY:

• Responsible for training and orienting new staff. May serve as a team leader or supervisor in a smaller research setting.

EEO Statement
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.

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